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Journal Article 3

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2016 2

2009 1

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Manihot esculenta 1

Group similarity http://dx.doi.org/10.1631/FITEE.1500187 1

Social tagging systems 1

Spectral clustering 1

Tag co-occurrence 1

core protein 1

expressed sequence tag 1

expression pattern 1

hepatitis B virus 1

pathway 1

tetracysteine tag 1

unigene 1

vector 1

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Construction and expression of hepatitis B virus vector encoding TC-tagged core protein

Yuanyuan LIN MD, Xiaoming CHENG MS, Yuhu SONG MD, Peiyuan LI MD, Ying CHANG MM, Jinjian YAO MD, Jusheng LIN MD, PhD, Li ZHOU PhD, Leiming XU PhD,

Frontiers of Medicine 2009, Volume 3, Issue 4, Pages 396-402 doi: 10.1007/s11684-009-0056-z

Abstract: HBV) particle, which is a compact virion with limited internal space, cannot be incorporated with GFP tagIt was recently reported that protein genetically inserted with a smaller size tetracysteine (TC) tagTC tag was genetically inserted near the immunodominant c/e1 site of HBV core protein by mutagenesis.Southern blot analysis showed that HBV virion formation was affected by the genetic insertion of TC tag

Keywords： hepatitis B virus vector tetracysteine tag core protein

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A social tag clustering method based on common co-occurrence group similarity

Hui-zong LI,Xue-gang HU,Yao-jin LIN,Wei HE,Jian-han PAN

Frontiers of Information Technology & Electronic Engineering 2016, Volume 17, Issue 2, Pages 122-134 doi: 10.1631/FITEE.1500187

Abstract: Tag clustering can aggregate tags with similar semantics together, and help mitigate the above problems

Keywords： Social tagging systems Tag co-occurrence Spectral clustering Group similarity http://dx.doi.org/10.1631

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Annotation and validation of genes involved in photosynthesis and starch synthesis from a

Yang ZHANG,Xin CHEN,Haiyan WANG,Zhiqiang XIA,Peng LING,Wenquan WANG

Frontiers of Agricultural Science and Engineering 2016, Volume 3, Issue 4, Pages 308-320 doi: 10.15302/J-FASE-2016113

Abstract: A full-length cDNA library from leaf and root tissues of cassava ( ) Arg7 and one accession of its wild ancestor W14 ( ssp. ) has been constructed. The library is comprised of four sub-libraries, containing 32640 recombinant clones, 6028 cDNA clones from their 5′ ends, and 128 clones from the 3′ ends were sequenced. In total, 5013 high-quality expressed sequence tags (ESTs) and 1259 unigenes were obtained. Of these, 746 unigenes were identified by their sequence homologies to ESTs from model plants, and 323 unigenes were mapped onto 114 different KEGG pathways. From these, 24 differentially expressed genes involved in starch metabolism and photosynthesis were identified and five of them were selected to compare their expression level between Arg7 and W14. Notably, Arg7 has a higher net photosynthesis rate in leaves, higher ribulose-1,5-bisphosphate carboxy-lase oxygenase activities in leaves, and higher AGPase activity in roots. This resource is the first EST collection from wild cassava and should be of value for gene discovery, genome annotation and studies of evolution.

Keywords： Manihot esculenta expressed sequence tag unigene pathway expression pattern